RBS

Part:BBa_K4951026:Design

Designed by: Julia Vu   Group: iGEM23_Stanford   (2023-10-08)


KanR Riboswitch 13


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The preceding homology arms were designed to correspond with the region in which we intended to insert the toehold (between the T7 promoter and the open reading frame of gene 2.5). The anterior homology arms were designed to correspond with the sfGFP gene to allow us to test the switches in a cell-free environment using the fluorescent gene as a reporter to quantitatively measure the expression level with and without the trigger sequence.


Source

This part was generated with novel riboregulator design software designed by Chritian Choe, a graduate student from the Das Lab at Stanford Unversity, against the KanR gene derived from Twist Biosciences. The exposed regions on the KanR folding structure make it an ideal antibiotic-resistance gene to target for the development of our toehold switches.

References